Grow the microbes the petri dish never could.

A patented, ready-to-use 3D hydrogel that reproduces the physical and chemical microenvironment of a specific human mucus or biofilm: oxygen gradients, viscoelasticity (G′ > G″), structure and nutrients. It ships standardized, in multi-well plates or syringes, so you inoculate and run without building a matrix yourself.

Aerobes, facultative anaerobes and strict anaerobes, including fastidious and low-abundance species that fail in standard 2D culture. The Universal Bac³Gel supports all ESKAPE pathogens (Gram-positive and Gram-negative), and the site-specific models are tuned to their native communities: gut, vaginal and airway.

Dissolve the gel with the Bac³Gel dissolution medium (50 mM sodium citrate) to release the cells intact. You can then perform CFU counts, 16S / shotgun sequencing, metabolomics or imaging, fully compatible with your existing workflow.

Yes. The biofilm-relevant matrix lets you determine MIC, MBC and MBEC, and run full antibiofilmograms against antibiotic panels and combinations. Because biofilm cells tolerate antibiotics far above the planktonic MIC, testing in 3D gives results that predict real-world performance.

The standardized multi-well plate format works with standard plate readers, and the gels are transparent enough for confocal and fluorescence microscopy to resolve 3D biofilm architecture in situ, with no need to disrupt the sample.

Yes, that is a core aim. By reproducing human mucus microenvironments in vitro, the models provide human-relevant data for colonization, permeability, efficacy and toxicity studies without animals, aligning with 3R and regulatory principles.

The matrix hydrates in NaCl, Mueller-Hinton, Luria-Bertani or your own medium of choice, so you keep the biochemical conditions your assay requires.

Book an introductory call. Tell us your niche, organism and readout; we’ll recommend a model, share the protocol pack and ship a starter plate to your lab.